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Original Research Article | OPEN ACCESS

Effects of Xinwei granule on expression levels of cyclin D1 and its upstream genes in gastric intraepithelial neoplasia tissues

Jingbin Wang1,2, Cunguo Yu3,4 , Xinyao Liu2, Yang Zhang1, Guoying Liang1, Zhaoxia Liu1

1Department of Spleen and Stomach Disease, Heilongjiang University of Chinese Medicine, Haerbin, Heilongjiang Provence 150040; 2Department of Spleen and Stomach Disease, Guangzhou University of Chinese Medicine-Shenzhen Hospital, Shenzhen, Guangdong Province 518000; 3College of Electrical Engineering, Yanshan University, Qinhuangdao, Hebei Province 066004, China; 4Department of Chinese Medicine, Qinhuangdao Haigang Hospital, Qinhuangdao, Hebei Province 066000, China.

For correspondence:-  Cunguo Yu   Email: yucunguocgc@163.com   Tel:+863353267453

Accepted: 27 February 2019        Published: 31 March 2019

Citation: Wang J, Yu C, Liu X, Zhang Y, Liang G, Liu Z. Effects of Xinwei granule on expression levels of cyclin D1 and its upstream genes in gastric intraepithelial neoplasia tissues. Trop J Pharm Res 2019; 18(3):485-490 doi: 10.4314/tjpr.v18i3.6

© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To explore the effects of Xinwei granule (XWG) on low-grade gastric intraepithelial neoplasia (LGIN) and the underlying mechanisms.
Methods: To establish LGIN model, Wistar rats were treated with N-methyl-N'-nitrosoguanidine for 3 months. LGIN model rats were randomly grouped into five groups (n = 15), viz, negative control (NC), normal saline (NS) group, Xinwei granule (XWG) group, Weifuchun tablet (WFCT) group, and vatacoenayme tablet (VT) group. Normal rats (n = 17) served as negative control. Histological evaluation of gastric mucosa was undertaken using hematoxylin and eosin staining. Quantitative real-time polymerase chain reaction (qRT-PCR), western blot, and immunohistochemical assays were performed to determine mRNA expressions, protein expression, and the distribution of cyclin D1, kruppel-like factor 4 (KLF4), and p21-WAF1-CIP1, respectively.
Results: Compared with LGIN group, the body weight of the rats increased in XWG, WFCT, and VT groups. The pathological characteristics of LGIN group were alleviated by XWG, WFCT and VT treatments. The positive expression of cyclin D1 was enhanced in LGIN group, but reduced in XWG, WFCT and VT groups. The expression levels of KLF4 and p21-WAF1-CIP1, upstream regulators of cyclin D1 reduced in LGIN groups. However, administration of XWG, WFCT and VT strengthened the expressions of KLF4 and p21-WAF1-CIP1. More importantly, the protective effects of XWG against LGIN were superior to those of WFCT and VT.
Conclusion: Xinwei granules alleviate LGIN in vivo by inhibiting cyclin D1 expression and enhancing KLF4 and p21-WAF1-CIP1 expression.

Keywords: Xinwei Granule, Cyclin D1, Kruppel-like factor 4, p21-WAF1-CIP1, Low-grade gastric intraepithelial neoplasia

Impact Factor
Thompson Reuters (ISI): 0.523 (2021)
H-5 index (Google Scholar): 39 (2021)

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